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Muscle fibres cultured in medium with insulin or albumin exclusively did not hypertrophy or change the number of sarcomeres in series. In the presence of albumin, all cultured muscle fibres were stable for at least 10 days. Caffeine contractures of in-excitable muscle fibres produced 80.4 ± 2.4% of initial peak tetanic force, indicating impaired excitation–contraction (E–C) coupling in in-excitable fibres. In culture medium with insulin, 50% of the muscle fibres became in-excitable within 7–12 days, whereas the other 50% were stable. The medium was supplemented with (final concentrations): (1) bovine insulin (6 nmol/L or 200–600 nmol/L), (2) 0.2% bovine albumin or (3) 0.2% bovine albumin in combination with insulin (120 nmol/L). Fibres were cultured in a serum-free medium at slack length (mean sarcomere length 2.3 μm) for 8 to 22 days. iliofibularis of Xenopus laevis and attached to a force transducer in a culture chamber. ![]() Single muscle fibres were dissected from m. The aim of this study was to investigate effects of albumin and insulin separately as well as in combination on mature muscle fibres during long-term culture. ![]() ![]()
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